Multiple Forms of α-Glucosidase From Bovine Spleen
نویسندگان
چکیده
منابع مشابه
beta-Glucosidase activator protein from bovine spleen ("coglucosidase").
fl-Glucosidase-stimulating proteins (“co-&glucosidase”) have been isolated from bovine spleen by acidification of homogenized spleen, heat denaturation, and chromatography with DEAE-Sephacel, Sephadex G-75, hydroxyapatite, and decyl agarose columns. Gel electrophoresis of the product revealed a trace of inert protein and two fast-moving bands, a major diffuse band and a minor, faster-moving ban...
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Preparations of cathepsin D from the bovine uterus contain at least 12 distinct forms of enzyme which digest hemoglobin at pH 3.2. Ten of these forms (2 through 11) have been purified by DEAE-Sephadex chromatography and disc electrophoresis. Six major forms (4, 5, 6, 7, 8, and 12) account for 94% of the total cathepsin D activity of the uterus. These six forms have been unequivocally identified...
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insulin has been suggested by Cuatrecasas & Illiano (197 1) on the basis of neuraminidase treatment of adipose-tissue cells. Enzymic digestion was reported to have no effect on the total quantity of insulin receptor in the membranes or on the affinity of the receptor for insulin, but abolished the effect of insulin on glucose transport and on lipolysis. As shown in Fig. 1, neuraminidase action ...
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The potent α-glucosidase inhibitor (compound I) was isolated from coffee brews by the activity-based fractionation and identified as a β-carboline alkaloid norharman (9H-pyrido[ 3.4-b]indole) on the basis of mass spectroscopy and nuclear magnetic resonance spectra ((1)H NMR, (13)C NMR, and COSY). The norharman showed a potent inhibition against α-glucosidase enzyme in a concentration dependent ...
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The α-glucosidase inhibitory activity of an aqueous extract and compounds from the aerial parts of V. corymbosa was demonstrated with yeast and rat small intestinal α-glucosidases. The aqueous extract inhibited yeast α-glucosidase with a half maximal inhibitory concentration (IC50) of 28.6 μg/mL. Bioassay-guided fractionation of the extract led to the isolation of several compounds, including o...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1972
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.36.483